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Photoprotection of Chloropyll by β-Carotene

 

This Video demonstrates an experiment showing the photoprotection of chlorophyll by β-Carotene. For this pumpkin seed oil is mixed 1:1 with acetone and applied to a filter paper. Under irradiation with UV light the chlorophyll shows an intensive red fluorescence.

After applying β-carotene solution in heptane to a small point on the filter paper, the fluorescence of the chlorophyll is reduced. In the next step the filter paper is irradiated in a large area around the spot with the β-carotene solution. The fluorescence of the chlorophyll in this area is almost completely gone, only the spot that had β-carotene applied to it shows the same fluorescence as before the filter paper was irradiated.